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uppP [2019-07-09 11:27:29]
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uppP [2019-07-09 11:27:29]

minor undecaprenyl pyrophosphate phosphatase
locus
BSU31150
pI
7.90
mw
30.19 kDa
protein length
276 aa Sequence Blast
gene length
831 bp Sequence Blast
function
synthesis of undecaprenyl phosphate as precursor for lipid II
product
minor undecaprenyl pyrophosphate phosphatase
essential
no
ec
3.6.1.27
synonyms
yubB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    3,194,635 3,195,465

    Phenotypes of a mutant

  • inactivation of ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' reduces sporulation efficiency to 0.4% that of wild type cells; distended intermembrane space [Pubmed|26735940]
  • a ''[gene|A58729F80A0BB6683648E472E385711A6DE4D373|bcrC]'' ''[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]'' double mutant is not viable (unless ''[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]'' is artificially expressed) [Pubmed|27528508]

    • The protein

    Catalyzed reaction/ biological activity

  • Undecaprenyl diphosphate + H2O = undecaprenyl phosphate + phosphate (according to Swiss-Prot)

    • Protein family

  • uppP family (according to Swiss-Prot)

    • Structure

  • [PDB|5OON] (from E. coli, 47% identity) [pubmed|29540682]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • Additional information

  • [protein|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|UppP] seems to be a minor undecaprenyl pyrophosphate phosphatase, the major enzyme is [protein|A58729F80A0BB6683648E472E385711A6DE4D373|BcrC] [Pubmed|22904278]

    • Expression and Regulation

    Operons

    genes
    [gene|5B769207B05D93FEC36E97BB7074F29BB290C55A|yubA]-[gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A224 (yubB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/224 NBRP B. subtilis, Japan]
  • BKE31150 ([gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTTGATTCTCCCCA, downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC
  • BKK31150 ([gene|5A7A79A11FCBA9C90DAF526B8EAB6791D7D88E99|uppP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTTGATTCTCCCCA, downstream forward: _UP4_TAAGTCGAAAAATAAGGCTC

    • References

  • 18763711,22904278,26735940,27528508,29259598,29540682